Abstract 134
Azhar Rashid Lone, Nalini Tiwari, Samrendra Singh Thakur, Oren Pearlson, Tomáš Pavlíček, Shweta Yadav, 2020: Exploration of four new Kanchuria sp. of Earthworms (Oligochaeta: Megascolecidae) from the North Eastern Region of India using DNA bar-coding approach. Journal of Asia-Pacific Biodiversity, https://doi.org/10.1016/j.japb.2020.02.004.
The present investigation was carried out on the earthworms collected from the reserve forests of Meghalaya, which is a part of the North Eastern Region (NER) of India, a hot spot of biodiversity to assess diverse earthworms. We adopted integrative approaches involving morpho-anatomical taxonomy and Cytochrome oxidase 1 (COI-1) sequences using DNA barcoding technique to discriminate different species of Kanchuria genus. The exploration revealed the presence of six Kanchuria species among which four are new; Kanchuria daribokgrensis sp nov, K. karorensis sp nov, K. makhulensis sp nov, and K. mohiskulensis sp nov. Moreover, the species of K. turaensis and K. octotheca were additionally identified. The maximum intraspecific and minimum interspecific divergence of six Kanchuria species recorded was 6.11% and 14.85% respectively. The neighbor-joining tree was constructed using the K2P substitution model and species discovery was achieved by ABGD and BIN analysis. The study presented the detailed descriptions and illustrations of six species including four new species of Kanchuria supplemented with their DNA barcodes from Meghalaya (NER) to facilitate future species identification.